An immunoglobulin test measures the level of certain immunoglobulins, or antibodies,
in the blood. Antibodies are proteins made by the immune system to fight antigens,
such as bacteria, viruses, and toxins.
The body makes different immunoglobulins to combat different antigens. For example,
the antibody for chickenpox isn't the same as the antibody for mononucleosis. Sometimes,
the body may even mistakenly make antibodies against itself, treating healthy organs
and tissues like foreign invaders. This is called an autoimmune disease.
The five subclasses of antibodies are:
Immunoglobulin A (IgA), which is found in high concentrations
in the mucous membranes, particularly those lining the respiratory passages and gastrointestinal
tract, as well as in saliva and tears.
Immunoglobulin G (IgG), the most abundant type of antibody, is
found in all body fluids and protects against bacterial and viral infections.
Immunoglobulin M (IgM), which is found mainly in the blood and
lymph fluid, is the first antibody to be made by the body to fight a new infection.
Immunoglobulin E (IgE), which is associated mainly with allergic
reactions (when the immune system overreacts to environmental antigens such as pollen
or pet dander). It is found in the lungs, skin, and mucous membranes.
Immunoglobulin D (IgD), which exists in small amounts in the
blood, is the least understood antibody.
IgA, IgG, and IgM are often measured together. That way, they can give doctors
important information about immune system functioning, especially relating to infection
or autoimmune disease.
Why It's Done
Once an antibody is produced against a specific antigen, the next time that antigen
enters the body, the immune system "remembers" its response and produces more of the
same antibodies. In that way, checking for the presence of specific immunoglobulins
in the blood can be helpful in diagnosing or ruling out infections or certain other illnesses.
Doctors also rely on the immunoglobulin test as one of the tools to help diagnose
immunodeficiencies (when the immune system isn't working properly). A person can be
born with an immunodeficiency or acquire it through infection, disease, malnutrition,
burns, or as a side effect of medicines. Doctors may suspect an immunodeficiency in
a child who experiences frequent or unusual infections.
Your doctor will tell you if any special preparations are required before this
test. On the day of the test, it may help to have your child wear a T-shirt
or short-sleeved shirt to allow easier access for the technician who will be drawing
A health professional will usually draw the blood from a vein. For an infant, the
blood may be obtained by puncturing the heel with a small needle (lancet). If the
blood is being drawn from a vein, the skin surface is cleaned with antiseptic, and
an elastic band (tourniquet) is placed around the upper arm to apply pressure and
cause the veins to swell with blood. A needle is inserted into a vein (usually in
the arm inside of the elbow or on the back of the hand) and blood is withdrawn and
collected in a vial or syringe.
After the procedure, the elastic band is removed. Once the blood has been collected,
the needle is removed and the area is covered with cotton or a bandage to stop the
bleeding. Collecting blood for this test will only take a few minutes.
What to Expect
Either method (heel or vein withdrawal) of collecting a sample of blood is only
temporarily uncomfortable and can feel like a quick pinprick. Afterward, there may
be some mild bruising, which should go away in a day or so.
Getting the Results
The blood sample will be processed by a machine. The results are commonly available
within a few days. If results suggest any abnormality, the doctor will likely perform
The immunoglobulin test is considered a safe procedure. However, as with many medical
tests, some problems can occur with having blood drawn, like:
fainting or feeling lightheaded
hematoma (blood accumulating under the skin causing a lump or bruise)
pain associated with multiple punctures to locate a vein
Helping Your Child
Having a blood test is relatively painless. Still, many children are afraid of
needles. Explaining the test in terms your child can understand might help ease some
of the fear.
Allow your child to ask the technician any questions he or she might have. Tell
your child to try to relax during the procedure, as tense muscles can make it harder
and more painful to draw blood. It also may help for your child to look away when
the needle is being inserted into the skin.
If You Have Questions
If you have questions about the immunoglobulin test, speak with your doctor. You
also can talk to the technician before the procedure.