C-peptide, like the hormone insulin, is produced in the pancreas. Both are released
simultaneously from the pancreas when the compound called proinsulin is split into
Insulin is responsible for regulating the body's glucose levels. Glucose, the body's
main source of energy, is a sugar that comes from foods.
After a meal, our bodies break down the foods we eat into glucose and other nutrients,
which are then absorbed into the bloodstream from the gastrointestinal tract. Glucose
levels in the blood rise after a meal and trigger the pancreas to make insulin and
release it into the blood. When insulin is released, so is C-peptide.
Insulin works like a key that opens the doors to cells and allows the glucose in.
Without insulin, glucose can't get into the cells and it stays in the bloodstream.
The most common cause of abnormal fluctuations in blood glucose is diabetes.
C-peptide, on the other hand, has no effect on blood sugar. It is, however, useful
as a marker of insulin production, since the pancreas typically releases C-peptide
and insulin in about equal amounts.
In general, high C-peptide levels are associated with increased insulin production,
while low C-peptide levels indicate decreased insulin production.
Why It's Done
The C-peptide test may be ordered to determine how much insulin is being made by
the pancreas. This information is useful because:
It can help doctors tell the difference between type 1 and type 2 diabetes. In
type 1 diabetes, the pancreas produces little or no insulin and little or no C-peptide.
In type 2 diabetes, C-peptide levels will typically be normal or high, as the pancreas
works harder to overcome insulin resistance (when the tissues become less sensitive
to the effects of insulin) by producing more insulin.
It can help find the cause of low blood sugar (hypoglycemia), including the incorrect
use of diabetes medications.
Your doctor will let you know if any special preparations are needed for this test.
Sometimes it's necessary for a child to avoid eating or drinking for 8 hours prior
to the test; in other cases, doctors may want to check levels at specific times, such
as timed intervals after food or glucose is given.
On the day of the test, having your child wear a T-shirt or short-sleeved shirt
can make things easier for your child and the technician who will be drawing the blood.
A health professional will usually draw the blood from a vein after cleaning the
skin surface with antiseptic and placing an elastic band (tourniquet) around the upper
arm to apply pressure and cause the veins to swell with blood. A needle is inserted
into a vein (usually in the arm inside of the elbow or on the back of the hand) and
blood is withdrawn and collected in a vial or syringe.
After the procedure, the elastic band is removed. Once the blood has been collected,
the needle is removed and the area is covered with cotton or a bandage to stop the
bleeding. Collecting blood for this test will only take a few minutes.
What to Expect
Collecting a blood sample is only temporarily uncomfortable and can feel like a
quick pinprick. Afterward, there may be some mild bruising, which should go away in
a day or so.
Getting the Results
The blood sample will be processed by a machine. The results are commonly available
in a few days.
The C-peptide test is considered a safe procedure. However, as with many medical
tests, some problems can occur with having blood drawn, such as:
fainting or feeling lightheaded
hematoma (blood accumulating under the skin causing a lump or a bruise)
pain associated with multiple punctures to locate a vein
Helping Your Child
Having a blood test is relatively painless. Still, many kids are afraid of needles.
Explaining the test in terms your child can understand might help ease some of the
Allow your child to ask the technician any questions he or she might have. Tell
your child to try to relax and stay still during the procedure, as tensing muscles
and moving can make it harder and more painful to draw blood. It also may help for
your child to look away when the needle is being inserted into the skin.
If You Have Questions
If you have questions about the C-peptide test, speak with your doctor. You can
also talk to the technician before the procedure.