Prolactin is a hormone produced by the pituitary gland, the pea-sized gland near
the base of the brain that controls metabolism, growth, and sexual development. Although
prolactin is produced in small amounts in both males and non-pregnant females, its
main role is to stimulate lactation (milk production) in females during pregnancy
and maintain milk supply during breastfeeding. A prolactin test measures the amount
of this hormone in the bloodstream.
In a woman who breastfeeds, the nursing baby's demand for milk actually controls
the mother's supply. When a baby sucks at the breast, the woman's pituitary gland
releases more prolactin into her blood, increasing her milk production. If a mother
doesn't breastfeed, her prolactin levels will return to normal shortly after giving
Sometimes, though, prolactin levels are elevated even if a woman isn't pregnant
or breastfeeding, or in a male. The most common cause is a prolactinoma, a usually
benign (not cancerous) prolactin-producing tumor of the pituitary gland.
Why It's Done
Doctors may order prolactin tests to help diagnose, or monitor treatment of, prolactinoma.
Symptoms of a prolactinoma include headaches, vision problems (if tumor growth is
causing pressure on an optic nerve), and galactorrhea (milk production outside pregnancy
or breastfeeding, or in a male).
The prolactin test also may be used as part of a work-up for irregular menstrual
periods, fertility problems, some types of thyroid or adrenal gland dysfunction, anorexia,
and polycystic ovarian syndrome.
All these conditions can be associated with altered prolactin levels. A number
of medications and drugs can also stimulate the pituitary gland to release more prolactin
into the blood.
No special preparations are needed for this test. However, since prolactin levels
vary throughout the day and night — they're highest during sleep, just after
waking up, following strenuous exercise, and during periods of emotional stress —
your doctor may request that the test be performed at a certain time of day (often
a few hours after waking up). You also should be sure that the doctor knows about
any medications or drugs your child may be taking, since some may increase prolactin
levels in the blood.
It may help to have your child wear a T-shirt or short-sleeved shirt on the day
of the test to make things faster and easier for the technician who will be drawing
A health professional will draw the blood from a vein after cleaning the skin surface
with antiseptic and placing an elastic band (tourniquet) around the upper arm to apply
pressure and cause the veins to swell with blood. A needle is inserted into a vein
(usually in the arm inside of the elbow or on the back of the hand) and blood is withdrawn
and collected in a vial or syringe.
After the procedure, the elastic band is removed. Once the blood has been collected,
the needle is removed and the area is covered with cotton or a bandage to stop the
bleeding. Collecting the blood for the test will only take a few minutes.
What to Expect
Collecting a blood sample is only temporarily uncomfortable and can feel like a
quick pinprick. Afterward, there may be some mild bruising, which should go away in
a day or so.
Getting the Results
The blood sample will be processed by a machine. The results are commonly available
in 1-2 days.
The prolactin test is considered a safe procedure. However, as with many medical
tests, some problems can occur with having blood drawn, including:
fainting or feeling lightheaded
hematoma (blood accumulating under the skin causing a lump or bruise)
pain associated with multiple punctures to locate a vein
Helping Your Child
Having a blood test is relatively painless. Still, many children are afraid of
needles. Explaining the test in terms your child can understand might help ease some
of the fear.
Allow your child to ask the technician any questions he or she might have. Tell
your child to try to relax and stay still during the procedure, as tensing muscles
and moving can make it harder and more painful to draw blood. It also may help if
your child looks away when the needle is being inserted into the skin.
If You Have Questions
If you have questions about the prolactin test, speak with your doctor. You can
also talk to the technician before the procedure.